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Brand Name : GDSBio
Model Number : N9031/N9032
Certification : /
Place of Origin : China
MOQ : 1 bag
Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram
Supply Ability : 100 Bag/Bags Per Day
Delivery Time : 8work days
Packaging Details : small package or bulk distribute or OEM
Cat. No. : N9031/N9032
Size : 150/300ul
Concentration : 10U/µl
Appearance : colourless solution
Group : In Vitro Diagnostic Products
Recommend use concentration : 0.15U/μl
Logo Printing : With Logo Printing
Transport Package : Packing
Production Capacity : 100 Bag/Bags Per Day
Storage Conditions : Store at -20°C
Lyticase
Components
| Component | N9031 | N9032 | 
| Lyticase | 150ul | 300ul | 
| Buffer | 10ml | 20ml | 
Recommend use concentration: 0.15U/μl
Store at -20°C
For research use only
Description
Yeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Applications
Biochem/physiol Actions
Lyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Definition of Activity Unit
One unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25°C, using a suspension of yeast as substrate in a 3 ml reaction mixture.
 
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