PCR NGS Library Construction UDI UMI Adapters Primers For Illumina K003-A K003-B K003-C K003-D

NGS Library UDI UMI Adapters Primers for Illumina K003-A, K003-B, K003-C, K003-D

【Product Name】

UDI UMI Adapters Primers for Illumina

【Cat. No. & Spec.】

K003-A, K003-B, K003-C, K003-D; 96 rxns each

【Product Description】

#K003 UDI UMI Adapter has a 6 nt random complementary sequence, allowing for the addition of 6 nt UMI (Unique Molecular Identifiers) at both ends of the library after adapters ligation. The UMI allows multiplex analysis for multiple samples simultaneously, reducing the detection rate of false positive variants and improving the sensitivity of detection for low frequency mutations. The pre-mixed 96 UDI Primers have 8 nt specific matching i5 and i7 UDI (Unique Dual Index) sequences, which can be independently double-checked by library amplification to meet the high-throughput sequencing mixing requirements and greatly reduce the sample index misassignment.

【Storage Condition & Shelf Life】

All reagents should be stored at -20°C. The product is valid for 12 months.

Do not premix Adapter, Ligation Buffer and DNA Ligase before use to avoid formation of excessive Adapter dimer.

【Scope of application】

This product is a special adapter primer kit for # K001 Fast DNA Library Prep Kit, which is applicable for Illumina platform.

【Components】

Sequence Information

UMI Adapter:

5’ -ACACTCTTTCCCTACACGACGCTCTTCCGATCTNNNNNN-s-T-3’

5’-p-NNNNNNAGATCGGAAGAGCACACGTCTGAACTCCAGTC-3’

N indicates that each Adapter has a 6 nt random complementary UMI sequence, -s- indicates a thio modification, and -p- indicates a phosphorylation modification.

UDI i5 PCR Primer:

5’-AATGATACGGCGACCACCGAGATCTACAC[i5]ACACTCTTTCCCTACACGACGCT

C-3’

UDI i7 PCR Primer:

5’-CAAGCAGAAGACGGCATACGAGAT[i7]GTGACTGGAGTTCAGACGTGTGCTCT-3’

[i5] refers to the 8 nt i5 index sequence and [i7] refers to the 8 nt i7 index sequence.

The i7 index in the table below is the index sequence in the primers, and the index sequence is reverse complementary when input/sequencing in the sample sheet.

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Illumina K003-B NGS Library Construction

Illumina K003-D NGS Library Construction

PCR NGS Library Construction UDI UMI Adapters Primers For Illumina K003-A K003-B K003-C K003-D Images